Coding

Part:BBa_K1441013:Design

Designed by: Matthew Brewer   Group: iGEM14_Georgia_State   (2014-10-10)


DNA ligase from Escherichia coli with His-tag INSERT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1449
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1663


Design Notes

Had to create primers specific to the prefix and sufix to remove the stop condons presnt in all iGEM biobricks.


Source

Source of this part is pSB1C3 kit plate part BBa_k525710

References